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Image Search Results
Journal: PLoS ONE
Article Title: B-cell phenotype and IgD-CD27- memory B cells are affected by TNF-inhibitors and tocilizumab treatment in rheumatoid arthritis
doi: 10.1371/journal.pone.0182927
Figure Lengend Snippet: The expression of B-cell differentiation (CD5), maturation (CD23) and calcium signaling (CD38) cell markers was analyzed on B-cells (frequency and median fluorescence intensity, MFI) to characterize B-cell phenotype in circulation in early RA (ERA) and established RA patients under methotrexate treatment. The effect of TNF-inhibitors and tocilizumab treatment on B-cell markers expression was also assessed in established RA patients at baseline and after an average of 8 months of treatment. Bars represent median values with interquartile range. Differences were considered statistically significant for p <0.05. Non-parametric Mann-Whitney test was used for comparisons between 2 independent groups. For paired samples (before and after treatment), the Wilcoxon signed-rank test was used.
Article Snippet: B cell activating factor (BAFF) (Bender MedSystems, Austria), B-lymphocyte chemoattractant (BLC) also known as C-X-C motif chemokine 13 (CXCL13) and the soluble form of
Techniques: Expressing, Cell Differentiation, Fluorescence, MANN-WHITNEY
Journal: PLoS ONE
Article Title: B-cell phenotype and IgD-CD27- memory B cells are affected by TNF-inhibitors and tocilizumab treatment in rheumatoid arthritis
doi: 10.1371/journal.pone.0182927
Figure Lengend Snippet: The serum levels of C-X-C motif chemokine 13 (CXCL13) (A) , soluble CD23 (sCD23) (B) and B cell activating factor (BAFF) (C) were quantified in early RA (ERA) and established RA patients under methotrexate treatment by ELISA. The effect of TNF-inhibitors and tocilizumab treatment in the production of these serological markers was also assessed in established RA patients at baseline and after an average of 8 months of treatment. Lines represent median values. Differences were considered statistically significant for p <0.05. Non-parametric Mann-Whitney test was used for comparisons between 2 independent groups. For paired samples (before and after treatment), the Wilcoxon signed-rank test was used.
Article Snippet: B cell activating factor (BAFF) (Bender MedSystems, Austria), B-lymphocyte chemoattractant (BLC) also known as C-X-C motif chemokine 13 (CXCL13) and the soluble form of
Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY
Journal: PLoS ONE
Article Title: B-cell phenotype and IgD-CD27- memory B cells are affected by TNF-inhibitors and tocilizumab treatment in rheumatoid arthritis
doi: 10.1371/journal.pone.0182927
Figure Lengend Snippet: The expression of cell markers (CD5, CD23, CD38, CD69, CD86 and CD95) was analyzed on B-cells (frequency and median fluorescence intensity, MFI) by flow cytometry in established RA patients before and after an average of 8 months of biologic therapy with TNF-inhibitors and/ or tocilizumab, based on whether the patients presented a low to moderate disease activity (DAS28<3.2) or high disease activity (DAS28>3.2) after treatment. Differences were considered statistically significant for p <0.05 (Wilcoxon signed-rank test).
Article Snippet: B cell activating factor (BAFF) (Bender MedSystems, Austria), B-lymphocyte chemoattractant (BLC) also known as C-X-C motif chemokine 13 (CXCL13) and the soluble form of
Techniques: Expressing, Fluorescence, Flow Cytometry, Activity Assay
Journal: American Journal of Reproductive Immunology
Article Title: Serum markers of B‐cell activation in pregnant women with atopic asthma
doi: 10.1111/aji.13414
Figure Lengend Snippet: Box (median with IQR) and whisker (10th and 90th percentiles), and before‐after plots of sCD23 serum levels in the peripheral blood of healthy nonpregnant women and those with atopic asthma (HNP and ANP), during the third trimester of pregnancy (HP and AP), and during the postpartum period at least 6 weeks after delivery (HP/PP and AP/PP). Differences between unpaired and paired groups were tested using the Mann‐Whitney U ‐test or Wilcoxon signed‐rank test, respectively (A). Correlations between sCD23 serum levels and CD24 Hi CD27 + B‐cells (B), CD24 Hi CD38 Hi B‐cells (C) and IL‐10 secreting B‐cells (D) in ANP women. For (B), (C) and (D), Spearman correlation coefficients, 95% confidence intervals, and p ‐values are indicated. HNP: n = 35; HP: n = 43; HP/PP: n = 42; ANP: n = 32; AP: n = 20; AP/PP: n = 12. * p ‐value <0.05; ** p ‐value <0.01; *** p ‐value <0.001. Shaded areas represent normal range. All data regarding healthy women was adapted from Lima et al.
Article Snippet: For sCD23 quantification, serum samples were analyzed with
Techniques: Whisker Assay, MANN-WHITNEY
Journal: American Journal of Reproductive Immunology
Article Title: Serum markers of B‐cell activation in pregnant women with atopic asthma
doi: 10.1111/aji.13414
Figure Lengend Snippet: Comparison of B‐cell activation markers in AP AMP and AP NAMP groups
Article Snippet: For sCD23 quantification, serum samples were analyzed with
Techniques: Comparison, Activation Assay